模式菌株/非模式菌株：非模式菌株培养基名称:VY/2 AGAR培养基成分:Baker's yeast 5.00 g Vitamin B12 0.50 mg
CaCl2 x 2 H2O 1.36 g Agar (Difco) 15.00 g
Distilled water 1000.00 ml
Sterilize vitamin B12 separately by filtration. Prepare and store yeast cells as autoclaved stock suspension (5 g baker's yeast/100 ml distilled water, adjust pH to 6.5 and autoclave). Adjust pH of medium to 7.2 with KOH before, and after autoclaving and cooling to 50°C (use pH-indicator paper). For suspension of freeze-dried cells from ampoules add about 0.5 - 1.0 ml medium MD1 (per liter: casiton 3.0 g; calciumchloride dihydrate 0.7 g; magnesiumsulphate heptahydrate 2.0 g) to the vial with freeze dried material.
For DSM 11116 reduce amount of vitamin B12 to 0.05 mg/l.培养温度:30℃
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